FIG. 1.

Fungal loci and sequences related to this study. (A) Schematic representation of the fungal ribosomal gene cluster, with PCR primers indicated (arrows). (B) Pairwise alignment between fragments of the 18S rRNA gene from human and representative fungal species, showing the sequences of primers EF4 and 1536 (long boxes). The human sequence contains a 7-bp insertion within the region represented by primer EF4 (small box). K, A/G; Y, T/C. (C) Pairwise alignment between fragments related to the 5.8S rRNA gene and ITS2 from humans and Malassezia species, showing the sequence of primers Mal1F and Mal1R. Bold letters in the human sequences refer to conserved nucleotides with the fungal sequences. H, T/C/A.