FIG. 1.
Comparison of different probes in NV GGI RT-PCR. The real-time RT-PCR was performed on the Rotorgene 3000 platform with primers NV192 and NV193 and probe Ring1(a)/(b) (dashed lines) or NV-GGI-LNA (solid lines). Amplification plots of fluorescence intensities versus PCR cycle numbers are displayed for serial 10-fold dilutions of the NV GGI standard (2 × 102 to 2 × 104 NV copies per reaction). The end point measurement of the normalized fluorescence (Norm. Fluoro.) and CT values is shown in a tabular manner.