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Real-time RT-PCR of NV GGI standard. Amplification plots of fluorescence intensities versus PCR cycle numbers are displayed for serial 10-fold dilutions of the NV GGI standard (4 to 4 × 105 copies [cop.] per reaction). The NV GGI RT-PCR assay was performed on the LightCycler 1.2 with (A) FAM-labeled NV-G1-LNA (detection in F1 channel [530 nm]) and (B) DY521XL-labeled MS2 probe (detection in F3 channel [705 nm]). Neg. co., negative control.
