TABLE 2.

Sensitivity of NV GGI RT-PCR with and without MS2 IC

;2;;>Spiking titer (no. of NV GGI copies/ml)	;2;;>Corresponding no. of NV GGI copies/PCR	RT-PCR without MS2 IC			RT-PCR with MS2 IC
No. of subjects	No. of observed responses	Probita	No. of subjects	No. of observed responses	Probita
2.47 × 105	4,960	12	12	1.000	12	12	1.000
1.24 × 105	2,480	12	12	1.000	12	12	1.000
6.18 × 104	1,240	12	12	0.999	12	12	0.999
3.09 × 104	620	12	10	0.872	12	10	0.902
1.54 × 104	310	12	7	0.538	12	8	0.541
7.72 × 103	77	12	5	0.246	12	5	0.214
3.86 × 103	39	12	3	0.207	12	2	0.174
1.93 × 103	19	12	2	0.189	12	1	0.155
9.65 × 102	10	12	0	0.181	12	0	0.147

^aPredicted proportion of replicates positive from probit regression analysis (SPS5).