TABLE 2.
Primers used in amplification and sequencing of seven housekeeping loci for multilocus sequence typing analysis of the historical B. pseudomallei strain collection
| Locus | Primer name | Primer sequence (5′→3′) | Annealing temp (°C) | Applicationa |
|---|---|---|---|---|
| ace | ace-up | GCT CGG CGC TTC TCA AAA CG | 61 | Amp & Seq |
| ace-dn | CAT GTC CGT GCC GAT GTA GC | |||
| gltB | gltB-up | GGC GGC AAG TCG AAC ACG G | 61 | Amp & Seq |
| gltB-dn | GCA GGC GGT TCA GCA CGA G | |||
| gmhD | gmhD-up | CTC GCG CAG GGC ACG CAG T | 63 | Amp & Seq |
| gmhD-dn | GTC AGG AAC GGC GCG TCG TA | Seq | ||
| gmhD-dn(outer) | GGC TGC CGA CCG TGA GAC C | Amp | ||
| lepA | lepA-up | CGC TTG ATC GGC ACT GAA TGG | 63 | Amp & Seq |
| lepA-dn | CGA ACC ACG AAT CGA TGA TGA G | |||
| lipA | lipA-up | CAT ACG GTG TGC GAG GAA GC | 61 | Amp & Seq |
| lipA-dn | CAG GAT CTC GTC GGT CGT CT | |||
| narK | narK-up(outer) | GCC GCG CAC GAC CAG CGC | 62 | Amp |
| narK-up | CGG ATT CGA TCA TGT CCA CTT C | Seq | ||
| narK-dn | CGG CAC CCA CAC GAA GCC C | Amp & Seq | ||
| ndh | ndh-up | GCA GTT CGT CGC GGA CTA TC | 61 | Amp & Seq |
| ndh-dn | GGC GCG GCA TGA AGC TCC A |
a
Amp, amplification; Seq, sequencing; Amp & Seq, both.