TABLE 1.
Primer sequences for PCR-based AdV typing by fragment length analysis
| Primer (labeling)a | Oligonucleotide sequence (5′-3′) | Concn (μM) | Primer seminested PCR | Oligonucleotide sequence (5′-3′) | Concn (μM) |
|---|---|---|---|---|---|
| AdV A for (F) | CCAAAGGGCGCGCCYAATGC | 0.4 | |||
| AdV A rev 1 | CGAGCGTAGGAGCCATAGC | 0.2 | AdV A nested rev 1 | GCAGGGGTACATGGGTGTGG | 0.2 |
| AdV A rev 2 | CGGGCGTAAGACCCATAGC | 0.2 | AdV A nested rev 2 | GCACGGATACATGGGCGTAG | 0.2 |
| AdV B for 1 (F) | GCCCCAGCTTCAAACCCTAC | 0.2 | |||
| AdV B for 2 (F) | GTCCTAGCTTCAAGCCATAT | 0.2 | |||
| AdV B for 3 (F) | GTCCCAGTTTCAAACCCTAT | 0.2 | |||
| AdV B for 4 (F) | GCCCTAGCTTCAAGCCATAC | 0.2 | |||
| AdV B rev 1 | GTCTGGCAAATGAACCATAGC | 0.2 | AdV B nested rev 1 | CCATAGCAGGGTTTCATCTTTG | 0.2 |
| AdV B rev 2 | GTTTAGCAAAAGACCCATAGC | 0.2 | AdV B nested rev 2 | CCGTAGCAGGGTTTCATTTTAG | 0.2 |
| AdV B rev 3 | GCTTGGCGAAAGAACCATAGC | 0.2 | AdV B nested rev 3 | CCGTAGCATGGTTTCATCTTAG | 0.2 |
| AdV B rev 4 | GTTTGGCAAAGGACCCGTAGC | 0.2 | AdV B nested rev 4 | CCGTAGCATGGCTTCATGTTGG | 0.2 |
| AdV B rev 5 | GTCTCGCAAAAGACCCGTAGC | 0.2 | |||
| AdV C for (J) | ACTGCCTACAACGCTCTRGC | 0.4 | AdV C nested rev 1 | GTCTTTTTTAGTACTCTGCC | 0.2 |
| AdV C rev | CCRTAGCATGGTTTCATGGG | 0.4 | AdV C nested rev 2 | GTTTTTTTAAGCACTCTCCC | 0.2 |
| AdV C nested rev 3 | GTCTTTTTAAGAACCCTTCC | 0.2 | |||
| AdV C nested rev 4 | GTCTTTTTAAGGACTCTCCC | 0.2 | |||
| AdV E for (J) | CTGGCTCCCAAGGGAGCGCC | 0.4 | |||
| AdV E rev | AGTGTCCTTTAGAGCTCTGC | 0.4 | |||
| AdV F for (T) | TGCTGGATCGTGGTCCCAGC | 0.4 | AdV F nested rev 1 | CATGGCTGCATGGGCGTGG | 0.2 |
| AdV F rev | TGTTGGTTTAGCGTATGACC | 0.4 | AdV F nested rev 2 | CATGGTAACATTGGCGTGG | 0.2 |
a
Labeling: F, fluorescein; J, JO; T, TAMRA. for, forward; rev, reverse.