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. 2006 Aug;44(8):2884–2889. doi: 10.1128/JCM.02594-05

FIG. 2.

FIG. 2.

HAT-PCR-OC test principle. Test side. In case of a positive sample, during migration, the T. brucei gold probes hybridize with the T. brucei amplicons that will accumulate on the Neutralite avidin line on the test side of the stick. Control side. During migration the IC gold probes hybridize with IC amplicons that will accumulate on the Neutralite avidin line on the control side of the stick (control for PCR). The unbound IC gold probes hybridize with the complementary migration control probes (control for migration).