TABLE 2.

Appearances of colonies and zones of hemolysis for tested strains on four different blood agar plates

Strain and characteristica	Appearance of colonies and hemolysis on blood agar plates
	HBA	CSBA	DSBA	HuBA
S. pneumoniae ATCC 6305
Colony appearance	Shiny, grey	Mucoid, grey	Dull, grey	Dull, grey
Colony size (mm)	1	1	1	Pinpoint
Alpha-hemolysis	Small, <1 mm	Obvious, 1-5 mm	Obvious, 1 mm	Absent
S. pneumoniae ATCC 49619
Colony appearance	Shiny, mucoid grey	Dry, grey	Dry, grey	Shiny, grey
Colony size (mm)	1	1.5	1	Pinpoint
Alpha-hemolysis	Obvious, 1 mm	Obvious, 1-5 mm	Obvious, 1 mm	Absent
S. aureus ATCC 25923
Colony appearance	Opaque white glossy	Opaque white glossy	Opaque white glossy	Opaque white glossy
Colony size (mm)	1.5	1.5	1.5	1.5
Beta hemolysis	Faint, <1 mm	Obvious, 1 mm	Obvious, 1 mm	Absent
S. aureus ATCC 29213
Colony appearance	Opaque yellow glossy	Opaque yellow glossy	Opaque yellow glossy	Opaque yellow glossy
Colony size (mm)	1.5	1.5	1.5	1
Beta-hemolysis	Faint, <1 mm	Obvious, 1.8 mm	Obvious, 1.8 mm	Faint, <1 mm
S. pyogenes ATCC 19615
Colony appearance	Glossy white	Dry grey-white	Dry grey	Glossy white
Colony size (mm)	1	1-2	1	Pinpoint
Beta-hemolysis	Obvious, 5 mm	Faint, <1 mm	Obvious, 1 mm	Absent
S. pyogenes strain JC20
Colony appearance	Glossy white	Glossy white	Glossy white	Glossy white
Colony size (mm)	1.5-1.8	1	1	Pinpoint
Beta-hemolysis	Obvious, 1-5 mm	Obvious, 1 mm	Obvious, 1 mm	Absent

^aA clinical isolate of S. pneumoniae and a clinical isolate of S. aureus were also tested (results not shown).