TABLE 1.
Identification of 88 ascomycetous clinical yeast strains by PA, FC, and D1/D2 LSU rRNA gene sequencing
| Organisma or parameter | No. of strains identified by:
|
||
|---|---|---|---|
| D1/D2 LSU rRNA gene sequencing | PA | FCb | |
| C. albicans | 9 | 9 | 9 |
| C. bovina | 1 | 0c | ND |
| C. bracarensis | 1 | 0d | ND |
| C. dubliniensis | 4 | 0e | 4 |
| C. fermentati | 1 | 0f | 0f |
| C. glabrata | 30 | 30 | 30 |
| C. guilliermondii | 2 | 2 | 2 |
| C. kefyr | 1 | 1 | ND |
| C. krusei | 4 | 4 | 4 |
| C. lusitaniae | 2 | 2 | 2 |
| C. lusitaniae AF538871 | 3 | 0g | ND |
| C. palmioleophila | 1 | 0h | ND |
| C. parapsilosis | 9 | 9 | 9 |
| C. tropicalis | 12 | 12 | 12 |
| P. anomala | 1 | 1 | ND |
| S. cerevisiae | 7 | 0i | 7 |
| Total no. (%) correctly identified | 88 (100) | 81 (92) | 79 (90) |
Strains designated C. lusitaniae AF538871 represent isolates of C. lusitaniae with atypical D1/D2 sequences. Although they are phenotypically identical to other C. lusitaniae strains in commonly used tests, the D1/D2 sequence divergence of these strains prevents their detection with the standard C. lusitaniae probe. See text for discussion.
ND, species identity not determined because there was no probe for this species. Present probes do not distinguish between the closely related species C. fermentati and C. guilliermondii (16).
Isolate identified as C. inconspicua.
Isolate identified as C. glabrata.
Isolates identified as C. albicans.
Isolate identified as C. guilliermondii.
Isolate identified as C. lusitaniae.
Isolate identified as D. hansenii.
Isolates identified as Saccharomyces sp.