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Sensitivities of GBV-C RNA detection using different primer sets. RNA was extracted from GBV-C RNA positive control serum. The control RNA template was diluted 1:800 (initial dilution [1]) and in serial twofold dilutions as indicated. RT-PCR was performed on each dilution using the four designated primer sets. The NS3 and NS5A primers required 8- and 16-fold less RNA than did the 5′-NTR and E2 primers (respectively) to amplify GBV-C RNA.
