TABLE 2.

Gene fragments and oligonucleotide primers for amplification and sequencing

Locus	Gene product	GenBank accession no.	Fragment size (bp)	Primer typec	Primer sequence (5′ to 3′)
Cox1	Cytochrome oxidase	AY347307	419	F	ACATTTTCCACAAGGAAAAGGTGGA
				R	AACGAAAAACTCCAGAAGTACCTGA
AbaA	Transcription factor	AF272838	411	F	TTGGTCGGATGCATTGGA
				R	TAATCGATGGAGGTTCGGTA
CPE1	Catalase	AF537129	312	F	TCTCAACAGCTGGCCCGACA
				R	AAGGTGTGACCGCCGGCAA
NGS	NADH-dependent glutamate synthase	AL684347	425	F	TGAGAGAGTAACTGCCTCATCAGGA
R		GAGGACCGGCAACTTCCCTGA
ICL1	Isocitrate lyase	AF373018	435	F	TACCGACCTCCTTGCCATTGCA
				R	TTGACGGCGCATTGCGTACCA
LNS	Lovastatin nonaketide synthase	AL684236	454	F	CTCCGGGATCAACCAATGTACAGA
R		ATTCGGGATCAACCAATGTACAGA
PAA	Polyaromatic amino acid biosynthesis	AL684053	438	F	CGTGAAGGTGGCTTGCGCAA
R		GTCCTTGGGAACGCCAGGAA
StlA	Transcription factor	AF284062	459	F	CAAAAGTCACATTCCTGCCCTA
				R	GACTCTTCTTCGAGCGAACCAA
MP1	Cell wall antigen	AF009957	450a	F	CAAGCCCTCCAGAAAGGTATCCA
				R	CTTTGTGGAGACCAATTCGCTGA
			303b	F	GTTGGTGGGGGTTATGGTCCTCA
				R	GAGAAGCATCACCGCCCACGTA

^aPrimer types F and R correspond to positions 527 to 549 and positions 954 and 976, respectively, of MP1 gene sequence for P. marneffei isolate PM4 (8).

^bPrimer types F and R correspond to positions 386 to 408 and positions 698 and 710, respectively, of MP1 gene sequence for P. marneffei isolate PM4 (8).

^cF, forward; R, reverse.