TABLE 2.
Locus | Gene product | GenBank accession no. | Fragment size (bp) | Primer typec | Primer sequence (5′ to 3′) |
---|---|---|---|---|---|
Cox1 | Cytochrome oxidase | AY347307 | 419 | F | ACATTTTCCACAAGGAAAAGGTGGA |
R | AACGAAAAACTCCAGAAGTACCTGA | ||||
AbaA | Transcription factor | AF272838 | 411 | F | TTGGTCGGATGCATTGGA |
R | TAATCGATGGAGGTTCGGTA | ||||
CPE1 | Catalase | AF537129 | 312 | F | TCTCAACAGCTGGCCCGACA |
R | AAGGTGTGACCGCCGGCAA | ||||
NGS | NADH-dependent glutamate synthase | AL684347 | 425 | F | TGAGAGAGTAACTGCCTCATCAGGA |
R | GAGGACCGGCAACTTCCCTGA | ||||
ICL1 | Isocitrate lyase | AF373018 | 435 | F | TACCGACCTCCTTGCCATTGCA |
R | TTGACGGCGCATTGCGTACCA | ||||
LNS | Lovastatin nonaketide synthase | AL684236 | 454 | F | CTCCGGGATCAACCAATGTACAGA |
R | ATTCGGGATCAACCAATGTACAGA | ||||
PAA | Polyaromatic amino acid biosynthesis | AL684053 | 438 | F | CGTGAAGGTGGCTTGCGCAA |
R | GTCCTTGGGAACGCCAGGAA | ||||
StlA | Transcription factor | AF284062 | 459 | F | CAAAAGTCACATTCCTGCCCTA |
R | GACTCTTCTTCGAGCGAACCAA | ||||
MP1 | Cell wall antigen | AF009957 | 450a | F | CAAGCCCTCCAGAAAGGTATCCA |
R | CTTTGTGGAGACCAATTCGCTGA | ||||
303b | F | GTTGGTGGGGGTTATGGTCCTCA | |||
R | GAGAAGCATCACCGCCCACGTA |
Primer types F and R correspond to positions 527 to 549 and positions 954 and 976, respectively, of MP1 gene sequence for P. marneffei isolate PM4 (8).
Primer types F and R correspond to positions 386 to 408 and positions 698 and 710, respectively, of MP1 gene sequence for P. marneffei isolate PM4 (8).
F, forward; R, reverse.