FIG. 1.

Production of the recombinant p30 produced in T. ni larvae. Fourth-instar larvae were inoculated with different doses of recombinant p30BAC, and infections were allowed to progress for 48, 72, or 96 h postinfection. Total protein extracts from each group were analyzed by (a) capillary electrophoresis: gray bars represent the percentage of the p30r protein produced out of the total extracted protein, and black bars indicate the mortality rate associated with each group; (b) Coomassie brilliant blue staining of SDS-page gels; and (c) Western blotting using a pool of swine sera from ASFV-seropositive animals as a probe. Arrows indicate the position of the recombinant p30.