TABLE 1.

Different supplemented RPMI 1640 broths evaluated for susceptibility testing of Malassezia spp.^a

Medium	Supplementb									Medium property(ies)
	Tween 40 (0.5%)	Glycerol (1 %)	Tween 80 (0.05%)	Oleic acid (0.5%)	Peptone (1%)	Glucose (1.8%)	Olive oil (1%)	Ox bile (0.5%)	Malt extract (0.5%)
1	+	+		+						Hazy
2			+	+						Hazy
3		+	+	+						Turbid
4		+	+							Clear; middle-sized button growth (++)
5			+							Clear; small button growth (+)
6		+								Clear; small button growth (+)
7		+	+		+	+				Clear; larger button growth (+++) but optimal growth of only M. furfur
8		+	+		+	+	+			Hazy
9		+	+		+	+		+	+	Larger button growth (+++) but turbid medium due to ox bile precipitation

^aEach of the nine medium formulations (pH 7.0 for each) was evaluated by testing each reference isolate of the six species evaluated.

^b+, supplement added to RPMI 1640 medium.