FIG. 2.

Novel HPV testing algorithm. DNA was isolated from cervical scrapes by use of the MagNA Pure LC instrument and subjected to both β-globin PCR and SPF₁₀ PCR. The PCR products are analyzed by a DNA enzyme immunoassay with specific probes. If the β-globin PCR-DEIA yielded a negative result, the PCR was repeated. If the SPF₁₀ PCR-DEIA yielded a negative result, the sample was considered HPV negative. Samples with borderline DEIA results were retested. Amplimers from SPF₁₀ PCR-DEIA-positive samples were tested by LiPA (version 1.0, which contains probes for 25 genotypes) to determine the HPV genotype. If the LiPA yielded a negative result, SPF₁₀ amplimers were sequenced. All samples HPV positive by SPF₁₀ PCR-DEIA were also tested by TS16 and TS18 PCRs.