TABLE 1.

Detection of HPV genotypes in mixtures containing different ratios of input DNA from two HPV genotypes

HPV type in sample	Genotype ratio (no. of genome copies/PCR mixture)	PCR-DEIA result			HPV type(s) by SPF10 LiPA
		SPF10	TS16	TS18
11	100	+	NDa	-	11
11 and 18	100:100	+	ND	+	11 + 18
11 and 18	1,000:100	+	ND	+	11 + 18
11 and 18	10,000:100	+	ND	+	11 + 18
11 and 18	100,000:100	+	ND	+	11
11 and 18	1,000,000:100	+	ND	+	11
11 and 18	10,000,000:100	+	ND	+	11
44	100	+	-	ND	44
44 and 16	100:100	+	+	ND	16 + 44
44 and 16	1,000:100	+	+	ND	16 + 44
44 and 16	10,000:100	+	+	ND	44
44 and 16	100,000:100	+	+	ND	44
44 and 16	1,000,000:100	+	+	ND	44
44 and 16	10,000,000:100	+	+	ND	44
16	100	+	+	-	16
18 and 16	100:100	+	+	+	16 + 18
18 and 16	1,000:100	+	+	+	16 + 18
18 and 16	10,000:100	+	+	+	16 + 18
18 and 16	100,000:100	+	+	+	18
18 and 16	1,000,000:100	+	+	+	18
18 and 16	10,000,000:100	+	+	+	18
18	100	+	-	+	18
16 and 18	100:100	+	+	+	16 + 18
16 and 18	1,000:100	+	+	+	16 + 18
16 and 18	10,000:100	+	+	+	16 + 18
16 and 18	100,000:100	+	+	+	16 + 18
16 and 18	1,000,000:100	+	+	+	16
16 and 18	10,000,000:100	+	+	+	16

^aND, not determined.