TABLE 3.
Result of PCR analysis using primers designed to amplify pBC218-specific sequencesa
| Forward primer | Reverse primer | Result for:
|
||||
|---|---|---|---|---|---|---|
| B. cereus03BB87 | B. cereus 03BB102 | B. cereus 03BB108 | B. cereusG9241 | B. anthracis 91-429C−2 | ||
| pBC218-ORF 0001a-F | pBC218-ORF 0001a-R | ++ | − | − | ++ | ++ |
| pBC218-ORF 0001b-F | pBC218-ORF 0001b-R | ++ | ft mb | − | ++ | ++ |
| pBC218-ORF 0001c-F | pBC218-ORF 0001c-R | ++ | − | − | ++ | ++ |
| pBC218-ORF 0005-F | pBC218-ORF 0005-R | ++ | − | − | ++ | ++ |
| pBC218-ORF 0012-F | pBC218-ORF 0012-R | ++ | − | ++ | ++ | − |
| pBC218-ORF 0029-F | pBC218-ORF 0029-R | ++ | − | − | ++ | − |
| pBC218-ORF 0031-F | pBC218-ORF 0031-R | ++ | − | − | ++ | 2 kb |
| pBC218-ORF 0043-F | pBC218-ORF 0043-R | ++ | − | − | ++ | mb/1 kb |
| pBC218-ORF 0047-F | pBC218-ORF 0047-R | ++ | − | − | ++ | − |
| pBC218-ORF 0055F | pBC218-ORF 0055R | ++ | − | − | ++ | − |
| pBC218-ORF 0059-F | pBC218-ORF 0059-R | ++ | − | − | ++ | − |
| pBC218-ORF 0064-F | pBC218-ORF 0064-R | ++ | ++ | − | ++ | − |
| pBC218-ORF 0067-F | pBC218-ORF 0067-R | ++ | − | − | ++ | − |
| pBC218-ORF 0077-F | pBC218-ORF 0077-R | ++ | ++ (ft) | − | ++ | ++ |
| pBC218-ORF 0086-F | pBC218-ORF 0086-R | ++ | − | − | ++ | − |
| pBC218-ORF 0089-F | pBC218-ORF 0089-R | ++ | − | − | ++ | − |
| pBC218-ORF 0096-F | pBC218-ORF 0096-R | ++ | ++ | ++ | ++ | − |
| pBC218-ORF 0099-F | pBC218-ORF 0099-R | ++ | − | − | ++ | − |
| pBC218-ORF 0111-F | pBC218-ORF 0111-R | ++ | − | ++ | ++ | 400 bp |
| pBC218-ORF 0119-F | pBC218-ORF 0119-R | ++ | − | − | ++ | − |
| pBC218-ORF 0122-F | pBC218-ORF 0122-R | ++ | − | − | ++ | − |
| pBC218-ORF 0129-F | pBC218-ORF 0129-R | ++ | ++ | ++ | ++ | ++ |
| pBC218-ORF 0134-F | pBC218-ORF 0134-R | ++ | − | − | ++ | − |
| pBC218-ORF 0139-F | pBC218-ORF 0139-R | ++ | − | ++ | ++ | − |
| pBC218-ORF 0141-F | pBC218-ORF 0141-R | ++ | − | − | ++ | − |
| pBC218-ORF 0147-F | pBC218-ORF 0147-R | mb | − | − | mb | − |
| pBC218-ORF 0150-F | pBC218-ORF 0150-R | ++ | − | − | ++ | − |
| pBC218-ORF 0157-F | pBC218-ORF 0157-R | ++ | − | − | ++ | − |
| pBC218-ORF 0165-F | pBC218-ORF 0165-R | ++ | − | − | ++ | − |
| pBC218-ORF 0171-F | pBC218-ORF 0171-R | ++ | − | − | ++ | − |
| pBC218-ORF 0180-F | pBC218-ORF 0180-R | ++ | − | − | ++ | ++ |
| pBC218-ORF 0184-F | pBC218-ORF 0184-R | ++ | − | − | ++ | ++ |
Columns 1 and 2 list the primers used to amplify different regions of the plasmid pBC218 from B. cereus G9241. Columns 3 to 7 show results of PCR experiments using these primers and different Bacillus isolate DNA as template. The two columns in boldface show results using B. cereus G9241 and B. cereus 03BB87 DNA as templates. Note that the results are identical for these two isolates. The sequences of the different primers used in this analysis are shown in Table S1 in the supplemental material. mb, multiple DNA fragments amplified; ft mb, faint multiple DNA fragments amplified; ft, faint amplicon. Where an amplicon size is shown, an amplicon of that size was produced and it is not the expected amplicon size based on the published pBC218 sequence.