TABLE 2.

Evaluation of PNA probe specificity

Organism	Source	MTBCCy3a		MAVTAMRAb		MKATAMRAc		MLEPTAMRAd		BacUni-1Fluo resulte
		Resultf	MMg	Result	MM	Result	MM	Result	MM
M. abscessus	DSM 44196	n	4	n	6	n	2	n	7	+-+++
M. avium subsp. avium	DSM 44156	n	4	+-+++	0	n	2	n	2	+-+++
M. avium subsp. avium	ATCC 15769	n	4	+-+++	0	n	2	n	2	+-+++
M. bovis	Clinical isolateh	+-+++	0	n	4	n	2	n	5	+-++
M. celatum	DSM 44243	n-(+)	1 (pos. 8)	n	5	n	2	n	7	+-++
M. chubuense	DSM 44219	n	1 (pos. 5)	n	5	n	3	n	5	+-+++
M. flavescens	Clinical isolate	n	4	n	6	n	3	n	4	+-++
M. fortuitum subsp. fortuitum	DSM 46621	n	4	n	6	n	2	n	8	+-+++
M. gordonae	DSM 44160	n	3	n	3	n-(+)	1 (pos. 8)	n	5	+-+++
M. haemophilum	DSM 44634	n	4	n-(+)	1 (pos. 6)	n	2	n-+	1 (pos. 9)	+-++
M. interjectum	DSM 44064	n	4	n	2	n	2	n	2	+-+++
M. intermedium	DSM 44049	n	4	n	3	n-(+)	1 (pos. 7)	n	3	+-+++
M. intracellulare	DSM 43223	n	4	n	3	n	2	n	3	+-+++
M. kansasii	DSM 44162	n	4	n	4	+-+++	0	n	4	+-+++
M. marinum	DSM 44344	n-(+)	1 (pos. 2)	n	5	n	2	n	7	+-+++
M. nonchromogenicum	Clinical isolate	n	4	n	4	n	3	n	5	+-++
M. phlei	Clinical isolate	n	4	n	6	n	2	n	5	+-++
M. scrofulaceum	DSM 43992	n	4	n	4	n	2	n	4	+-+++
M. septicum	Clinical isolate	n	4	n	6	n	3	n	4	+-++
M. simiae	Clinical isolate	n	4	n	4	n	2	n	4	(+)-++
M. smegmatis	DSM 43756	n	4	n	7	n	3	n	9	+-++
M. terrae	Clinical isolate	n-(+)	1 (pos. 8)	n	5	n	2	n	4	+-++
M. triviale	Clinical isolate	n	4	n	3	n	2	n	4	+-++
M. tuberculosis	H37Rv	+-++++	0	n	4	n	2	n	5	+-++
M. vaccae	Clinical isolate	n	4	n	6	n	3	n	4	+-++
M. xenopi	DSM 43995	n-(+)	4	n	5	n	2	n	6	+-+++

^aM. tuberculosis complex-specific PNA probe.

^bM. avium-specific PNA probe.

^cM. kansasii-specific PNA probe.

^dM. leprae-specific probe.

^eEubacterial probe.

^fn = negative. Fluorescence signal intensities: (+), slightly above background fluorescence; +, clearly visible; ++, strong; +++, very strong.

^gMM, number of mismatches; pos., position from 5′ end of probe.

^hFrom a clinical specimen; identification by 16S rRNA gene sequencing.