FIG. 1.
Agarose gel electrophoresis stained with ethidium bromide of stx2 fragments obtained by PCR from the STAH strain. (A) stx2 PCR, 346 bp. Lanes: S, STAH lysate; M, 100-bp DNA molecular marker (Promega); C, E. coli O157:H7 EDL933 lysate (stx2/stx1 positive control). (B) stx2 PCR, 215 bp. Lanes: C, E. coli K-12 C600 933W lysate (stx2 positive control); M, 100-bp DNA molecular marker (Gibco); L, STAH lysate; P, phage genome DNA; E, DNA obtained by plasmidic extraction.