TABLE 2.
Chromosomal regions of S. enterica serovars Typhimurium LT2, Typhi CT18, and Enteriditis (PT4) used to create primers for multiplex PCRa
| Assay | NCBI accession no. | Primer | Reaction concn (pM) | Primer sequence (5′→3′) | Amplicon size (bp) |
|---|---|---|---|---|---|
| STM 1 | AE008729 | STM0716F | 1 | AACCGCTGCTTAATCCTGATGG | 187 |
| STM0716R | 1 | TGGCCCTGAGCCAGCTTTT | |||
| STM 2 | AE008758 | STM1350F | 3 | TCAAAATTACCGGGCGCA | 171 |
| STM1350R | 3 | TTTTAAGACTACATACGCGCATGAA | |||
| STM 3 | AE008735 | STM0839F | 1 | TCCAGTATGAAACAGGCAACGTGT | 137 |
| STM0839R | 1 | GCGACGCATTGTTCGATTGAT | |||
| STM 4 | AE008913 | STM4525F | 1 | TGGCGGCAGAAGCGATG | 114 |
| STM4525R | 1 | CTTCATTCAGCAACTGACGCTGAG | |||
| STM 5 | AE008913 | STM4538F | 2 | TGGTCACCGCGCGTGAT | 93 |
| STM4538R | 2 | CGAACGCCAGGTTCATTTGT | |||
| STY 1 | AL627266 | STY0311F | 0.8 | TGGTATGGTTAAGCGGAGAATGG | 301 |
| STY0312R | 0.8 | GAGAGTCATAGCCCACACCAAAG | |||
| STY 2 | AL627273 | STY0346F | 0.8 | GGCTGGAGCAGCCTTACAAAA | 262 |
| STY0347R | 0.8 | AAGAGTTGCCTGGCTGGTAAAA | |||
| STY 3 | AL627273 | STY2299F | 3 | AATCCCCCCCCCTCAAAAA | 220 |
| STY2300R | 3 | GGTACACGTTTACTGTTTGCTGGA | |||
| STM 6 | AE008879 | STM3845F | 0.8 | ATATCTCATCGTCTCCTTTTCGTGT | 181 |
| STM3845R | 0.8 | GAAGGTCCGGATAGGCATTCT | |||
| STY 4 | AL627273 | STY2349F | 1 | AATTACGGAGCAGCAGATCGAGG | 124 |
| STY2349R | 1 | TGCGGCCAGCTGTTCAAAA | |||
| PT4 | AF370716 | PT4 F | 4 | GGCGATATAAGTACGACCATCATGG | 225 |
| PT4R | 4 | GCACGCGGCACAGTTAAAA | |||
| STM 7 | AE008795 | STM2150F | 4 | CATAACCCGCCTCGACCTCAT | 101 |
| STM2150R | 4 | AGATGTCGTGAGAAGCGGTGG |
a
The chromosomal regions of S. enterica serovars Typhimurium LT2 (STM), Typhi CT18 (STY), and Enteriditis (PT4) were used to create primers for multiplex PCR.