TABLE 2.
Comparison of MTBDR assay and sequencing results for the detection of mutations conferring resistance to rifampin for 76 RIF-resistant M. tuberculosis clinical isolates
| rpoB region | MTBDR result with:
|
Sequencing data (amino acid modification[s] in RpoB) | No. (%) of strains | |
|---|---|---|---|---|
| MUT WTa | MUTb | |||
| 509-514 | MUT WT1 | Q513K | 1 | |
| MUT WT1 | Q513P | 1 | ||
| 515-520 | MUT WT2 | MUT:D516V | D516V | 4 (5) |
| MUT WT2 | D516Y | 3 | ||
| MUT WT2 | M515I + D516Y | 2 | ||
| 521-525 | MUT WT3 | S522L | 2 | |
| 525-529 | MUT WT4 | MUT:H526D | H526D | 8 (10) |
| MUT WT4 | MUT:H526Y | H526Y | 6 (8) | |
| MUT WT4 | H526A | 1 | ||
| MUT WT4 | H526L | 3 | ||
| MUT WT4 | H526R | 3 | ||
| 530-534 | MUT WT5 | MUT:S531L | S531L | 36 (47) |
| MUT WT5 | S531W | 2 | ||
| MUT WT5 | S531T | 1 | ||
| MUT WT5 | L533P | 2 | ||
| MUT WT5+WT1 | S531C + L511P + F505L | 1 | ||
a
Absence of hybridization signal with the wild-type probes (WT1 to WT5).
b
Positive hybridization result with the specified mutant probe (MUT D516V, MUT H526D, MUT H526Y, or MUT S531L).