TABLE 2.
Percent agreement at optimal incubation times of either posaconazole or amphotericin B by Etest and YeastOne MICs with reference MICs and susceptibility data for the three methods for 126 Aspergillus isolatesa
| Species (no. of isolates tested) | Agent | % Agreement of reference with E/Yb | MICs (μg/ml) by:
|
|||||
|---|---|---|---|---|---|---|---|---|
| Etest
|
M38-A
|
YeastOne
|
||||||
| Range | G/MIC90 | Range | G/MIC90 | Range | G/MIC90 | |||
| A. flavus (18) | P | 100c/100d | 0.01-0.12 | 0.02/0.06 | 0.03-0.12 | 0.04/0.06 | 0.01-0.12 | 0.05/0.06 |
| A | 92c/94d | 0.5->8 | 4.1/>8 | 0.25-4 | 0.96/2 | 1.0-4 | 1.0/2 | |
| A. fumigatus (60) | P | 984/98d | <0.01-1.0 | 0.04/0.25 | 0.03-0.5 | 0.04/0.12 | 0.01-1.0 | 0.04/0.12 |
| A | 97c/92d | 0.25-8 | 1.45/2 | 0.12-4 | 0.67/2 | 0.25-2 | 0.58/1.0 | |
| A. nidulans (14) | P | 100c/100d | <0.01-0.03 | 0.01/0.03 | <0.01/0.06 | 0.03/0.06 | 0.01-0.25 | 0.05/0.12 |
| A | 93c/100d | 0.25->8 | 1.5/2 | 0.25-2 | 1.04/2 | 0.12/1.0 | 0.87/1.0 | |
| A. niger (16) | P | 904/100d | 0.12-0.5 | 0.21/0.5 | 0.06-0.25 | 0.06/0.25 | 0.03-0.12 | 0.08/0.12 |
| A | 100c/100d | 0.5-1.0 | 0.75/1.0 | 0.12-1.0 | 0.3/0.5 | 0.25-0.5 | 0.25/0.5 | |
| A. terreus (18) | P | 1004/100d | 0.06-0.12 | 0.06/0.06 | 0.03-0.06 | 0.03/0.03 | 0.03-0.12 | 0.06/0.12 |
| A | 100c/90d | 0.25-8 | 2.85/4 | 0.25-4 | 1.8/4 | 0.25-4 | 1.6/4 | |
| All Aspergillus spp. (126) | P | 97/99d | <0.015-1.0 | 0.068/0.25 | <0.01-0.5 | 0.04/0.12 | 0.01-1.0 | 0.056/0.12 |
| A | 96c/95d | 0.25->8 | 2.13/2 | 0.12-4 | 0.96/2 | 0.12-4 | 0.86/2 | |
a
NCCLS M38-A broth microdilution method; G, geometric mean MIC; MIC90, MICs for 90% of the isolates tested.
b
Percentage of agreement between MICs by reference (obtained at the recommended time of 48 h) and Etest (E) and between reference and YeastOne (Y); P, posaconazole; A, amphotericin B.
c
Highest percentage of agreement was obtained at 24 h (commercial method).
d
Highest percentage of agreement was obtained at 48 h.