TABLE 2.
Detection results from cultivation, species-specific PCR, and DGGE for the four pathogens associated with periodontitis in 25 subgingival plaque samples
| Species | No. of samples with positive results (sensitivitya [%])
|
||
|---|---|---|---|
| Cultivation | PCR | DGGE | |
| Actinobacillus actinomycetemcomitans | 3 (38) | 5 (100) | 8 (100/100) |
| Porphyromonas gingivalis | 9 (100) | 10 (100) | 9 (100/90) |
| Prevotella intermedia | 16 (88) | 16 (88) | 17 (94/88) |
| Tannerella forsythensis | 23 (100) | 24 (100) | 23 (100/96) |
| Treponema denticola | ND | ND | 12 |
a
The sensitivity of cultivation was calculated with DGGE as the reference standard. The sensitivity of PCR was calculated with cultivation as the reference standard. The sensitivity of DGGE was calculated with both cultivation and PCR as the reference standards, as indicated by the two sensitivity values, respectively, in parentheses. ND, not determined.