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SV fusion protein purification. (A) SDS-PAGE. (B) Western blot immunostained with anti-GST-Mex340 hyperimmune serum (1:10,000). (C) Western blot immunostained with anti-GroEL hyperimmune serum (1:10,000; Sigma). Lane 1, amylose affinity resin-purified MBP-Mex340-6xHis; lane 2, GroEL (B-PER wash); lane 3, amylose and Talon affinity resin-purified full-length MBP-Mex340-6xHis; lane 4, MBP2. MW, molecular weight marker (molecular weights in thousands).
