TABLE 2.

Specificities and sequences of probes used for Mycobacterium PCR-RLB assay

Probe	Strain	Melting temp (°C)	GenBank accession no.	Sequence
MYC	Mycobacterium genus	68.59	L15623	301-GTGGTGGGGTGTGGTGTTTG-320
ABS	M. abcessus	65.37	AJ314869	32-GGG AAC ATA AAG TAG GCA TCT GTA GTG-58
ASI	M. asiaticum	72.03	AF191087	35-TGC AGG CCG TGT GGA GTT CTC-55
AVI	M. avium	69.12	AJ314863	126-AAC ACT CGG TCC GTC CGT GT-145
CHE1	M. chelonae a/ba	62.81	AJ314873	33-GGA ACA TAA AGC GAG TTT CTG TAG-56
CHE2	M. chelonae c	67.75	AJ291584	55-AGT GGA TGC ATG CTT GGT GAA-75
DIE	M. diernhoferi	66.16	AF186463	221-GTT CGG ACG GTG TCT GTT GTT-241
FOR1	M. fortuitum a/c	66.11	AJ291587/89	89-ACA AAC TTT TTT GAC TGC CAG ACA C-113
FOR2	M. fortuitum b	72.55	AJ291588	112-TTT GCG GTG ATG GGA CTG CC-131
FOR3	M. fortuitum e	68.51	AJ291591	107-GAT TTT GCG GTG ATG GGA CC-126
FLA	M. flavescens	65.20	AJ291586	121-GGA ACA CTG CTT TGA GGA ATC AT-143
GAS	M. gastri	71.46	X97633	79-GTG CGC AAC AGC AAG CAA GC-98
GIL	M. gilvum	71.85	AJ314876	138-CGG TTT TCC GGG GTT GTG GT-157
GOR	M. gordonae	67.27	AJ315574	43-CGT GAG GGG TCA TCG TCT GTA-63
HAE	M. hemophilum	68.86	AY579400	130-CAC TCA GGC TTG TCC CAT GTT G-151
INT	M. intracellulare	69.21	AJ306711	133-GGT CGA TCC GTG TGG AGT CC-152
KAN1	M. kansasii a/b	66.72	L42263	213-GGG TGC GCA ACT GTA AAT GAA T-234
KAN2	M. kansasii c	67.74	L42264	150-AAA AGT GCC CCA ATT GGT GG-169
LEN	M. lentiflavum	69.65	AF318174	266-CCG ACT TTG GTC GAC GTG GT-285
LEP	M. leprae	65.48	X56657	172-TTG TCC CTC ATC TTT GGT GGT-192
MAL	M. malmoense	73.38	Y14184	126-AAC ACT CGG CCA GTC CGC GT-145
PHL1	M. phlei a	70.80	AJ291596	128-AAC ACC GTG TCG AGG ACT GCC-148
PHL2	M. phlei b	66.89	AJ291597	107-AAA CGT TTG TCG TTC GGT GG-126
SCR	M. scrofulaceum	67.40	AJ314884	129-ACT CGG CTC GTT CTG AGT GGT-149
SEN	M. senegalense	66.28	Y10385	55-GTG AGG AGT CTG TGC GCT GTA G-72
SHI	M. shimoidei	66.34	X99219	84-TGC ACA ACA ACA AGC GAG AAG-104
SIM	M. simiae	65.16	Y14186	138-ACT TCG GTT GAA GTG GTG TCC-158
SME	M. smegmatis	68.82	AJ291599	91-AAC GTT GAG ATG CGG TGT GGT-110
SZU	M. szulai	69.36	X99220	131-AGG CTT GGC CAG AGC TGT TGT-1513
TER	M. terrae	65.90	AJ314868	93-GTG CAG AGG AAT TAC GAA CAA CAA-116
TRI	M. triplex	69.21	AF214587	84-CAA CAG CAG ACA ATC GCC AGA C-105
MTBC	M. tuberculosis complex	68.04	AJ315568	79-TGC ATG ACA ACA AAG TTG GCC-99
ULC/MAR	M. ulcerans/M. marinum	61.52	X99217	124-AAC ATC TCT GTT GGT TTC GG-143
VAC	M. vaccae	72.60	AJ291600	134-AAT GCC GGC GAG GGA AAT CA-153
XEN	M. xenopi	69.39	AJ314866	66-TGT TGG GCA GCA GGC AGT AAC-86

^aIdentification of subtypes a and b of M. chelonae, M. fortuitum, M. kansasii, and M. phlei was based on sequence variations in their ITS regions.