TABLE 1.
Primer sequences for fluorescent (LUX) and unlabeled primers
| Primer name | Sequencea |
|---|---|
| L1 | FAM-caaccaCAGGGTATGATAGGCTATTGGtTG |
| L2 | JOE-gacattgTTGGATACTTGTGGTGCAATGtC |
| L4 | JOE-cacaaaGCCCCATCAATATCAGTTTGtG |
| L5 | FAM-cacaaaGCCCCATCAATATCAGTTTGtG |
| L1A | FAM-gtatccCGGCAATGCCATTGGAtAC |
| L2B | JOE-gacattgTTGGATACTTGTGGTGCAATGtC |
| L6 | FAM-caccttACGTCAAATGCGTGAAGGtG |
| L7 | JOE-catcaaTGCGTGAAGGTGAAGTTGAtG |
| L8 | FAM-caatccCCTTGTGAATCAAGTTCTGGAtTG |
| L9 | JOE-caatccCCTTGTGAATCAAGTTCTGGAtTG |
| L10 | JOE-caatcgTTGATGATTTACCAGTTCCGAtTG |
| L11 | FAM-caatcgTTGATGATTTACCAGTTCCGAtTG |
| L12 | JOE-cagcttCTGCGACAAGTGAATTGAAAGCtG |
| L13 | FAM-cagcttCTGCGACAAGTGAATTGAAAGCtG |
| L20 | JOE-gatctgTGAAGGGAATGCAGCAGAtC |
| L14 | FAM-gtacttcaCGGGATTTGCAGAATTTGAAGtAC |
| L18A | JOE-cacgcTAGGTGTTGTGCCTATGCGtG |
| L16 | FAM-cacctgTATCTCTAACGGCTTGTCAGGtG |
| L22 | JOE-caccgAACTTCTAATGACACTGGCGGtG |
| U1 | CGTATAAAAAGGACCAATTGGTCTG* |
| U2 | CGTATAAAAAGGACCAATTGGTCTA* |
| U3 | CGTATAAAAAGGACCAATTGGTCTT* |
| U4 | GTAAATCATCAACATCTGAAGATATG |
| U5 | GTAAATCATCAACATCTGAAGATGTG |
| U6 | GTAAATCATCAACATCTGAAGATGTA |
| U7 | GATCATCTTTATCTACTTCCACACGTGCA* |
| U8 | GATCATCTTTATCTACTTCCACACGTGCT* |
| U9 | ACCTACTAATCGCTCTCTCAAGTAA* |
| U10 | ACCTACTAATCGCTCTCTCAAGTAT* |
| U11 | TGCAGCACATTCAACAGAA |
| U12 | TGCAGCACATTCAACAGAC |
| U13 | TGCAGCACATTCAACAGAT |
| U14 | GATGAAGAAATTAACAAAAAAGCGCCT |
| U15 | GATGAAGAAATTAACAAAAAAGCGCCC |
| U16 | TTGCACAATCACCAAAGATGTATTATA* |
| U17 | TTGCACAATCACCAAAGATGTATTATT* |
| U18 | TTGAGCTGTCTTGGTTCATTGATT |
| U19 | AAACACTACTTGTTCCCGCTTCA |
| U20 | GGTCAGAGCCACGTTCACCA |
| U21 | TGCTTCAACATCCCAACCAA |
| U22 | TGGACACCGTCTTTATCAACGTCT |
| S1 | CAGGGTATGATAGGCTATTGGTTG |
| S2 | GCCCCATCAATATCAGTTTGTG |
| S3 | ACGTCAAATGCGTGAAGGTG |
| S4 | CCTTGTGAATCAAGTTCTGGATTG |
| S5 | TTGATGATTTACCAGTTCCGATTG |
| S6 | CTGCGACAAGTGAATTGAAAGCTG |
| aroE252GF | GGTATAATACAGATGGTATCGGTTATGTG |
| aroE252GR | ACCTGCGCCCAAAATTAAAA |
| spaF | AGCACCAAAAGAGGAAGACAA |
| spaR | GTTTAACGACATGTACTCCGT |
| mecAF | GATCGCAACGTTCAATTTAATTTTG |
| mecAR | GCTTTGGTCTTTCTGCATTCCT |
a
Boldface type indicates a polymorph at the SNP; lowercase type indicates hairpin loop structures of LUX primers. *, SNP is in reverse primer.