TABLE 4.
Allele-specific real-time PCR cycle times for known STsa
| Gene + polymorphb | CT for ST:
|
||
|---|---|---|---|
| 1 | 30 | 93 | |
| arcC210GCF | 19.30 | 28.26 | 31.62 |
| arcC210GTJ | 31.53 | 18.99 | 23.29 |
| arcC210GAF | Undet | 32.42 | 36.44 |
| tpi241 + 243ATGJ | Undet | 21.93 | 25.35 |
| tpi241 + 243GTGF | 26.84 | 16.97 | 20.72 |
| tpi241 + 243GTAJ | 19.11 | 26.60 | 30.13 |
| arcC162CTF | 18.44 | 28.28 | 19.07 |
| arcC162CAJ | 28.35 | 17.73 | 31.46 |
| gmk318AAJ | 23.78 | 21.7 | 24.51 |
| gmk318ATF | Undet | 20.96 | 20.37 |
| tpi36TJ | 30.45 | 21.92 | 20.93 |
| tpi36CF | 19.65 | 18.3 | 24.95 |
| pta294AAF | 20.75 | Undet | Undet |
| pta294ACJ | 24.38 | 20.07 | 18.88 |
| pta294ATF | 36.27 | 19.65 | 21.41 |
| pta383CTJ | 23.04 | 21.92 | 21.98 |
| pta383CAF | 32.47 | 19.82 | 21.38 |
a
Boldface type indicate reactions with perfectly matched allele-specific primers. Undet, undetermined.
b
5′ bases and subterminal mismatches are indicated. F, FAM labeling; J, JOE labeling.