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Secreted p-NPPC hydrolase activity of a mip plcA double mutant. Culture supernatants of 130b, AA108, NU268, and mip plcA double mutant NU317 were assayed for p-NPPC hydrolase activity. The data are the means and standard deviations of triplicate cultures and are representative of at least two other independent experiments. The differences in activity between NU317 and the other two mutants were significant (P < 0.05, as determined by Student's t test).
