FIG. 5.

NMB1220 and NMB0278 are able to block endocytosis and binding of known ligands to CHO cells transfected with human SR-A. (A) CHO WT, CHO hSR-AI, and CHO hSR-AII were preincubated with poly(I) or purified N. meningitidis proteins for 30 min before the addition of DiIAcLDL (5 μg/ml) and were maintained in their presence throughout the assay. The cells were washed and analyzed by flow cytometry. The graph shows the average increase in fluorescence from duplicate wells. (B) WT and hSR-AII cells were plated as described above and preincubated with Fucoidan, poly(I), or purified N. meningitidis proteins (100 μg/ml) for 30 min. Next, RdGnX-labeled, ethanol-fixed N. meningitidis was added (200 bacteria per cell for 1 h) while maintaining the inhibitor. The increase in fluorescence was measured by flow cytometry, and the average increase in fluorescence was plotted. The results are from a single assay that is representative of at least three similar experiments.