FIG. 2.

GFP-Rab4A recruitment to the chlamydial inclusion. (A to F) Three hours prior to infection with C. trachomatis serovar L2 at an MOI of approximately 20, HeLa cells transiently expressing GFP-Rab4A (A and D) were pretreated with nocodazole (NOC) (A to C) or BFA (D to F). Eight hours postinfection, cells were fixed and stained with antichlamydial LPS (B and E) and then viewed by LSCM. (C and F) GFP-Rab4A (green) and antichlamydial LPS (red) merged. (G to I) HeLa cells transiently expressing GFP-Rab4A (G) were infected with C. trachomatis serovar L2 at an MOI of approximately 20 and then incubated at 37°C for 24 h in the presence of chloramphenicol (CAM). Cells were then fixed and stained with antichlamydial LPS (H) and viewed by LSCM. (I) GFP-Rab4A (green) and antichlamydial LPS (red) merged. Small arrows indicate selected chlamydia-containing vacuoles that colocalize with GFP-Rab4A. Large arrows indicate chlamydia-containing vacuoles that do not colocalize with GFP-Rab4A. Bar, 10 μm.