Skip to main content
. 2006 Sep;74(9):5236–5243. doi: 10.1128/IAI.00329-06

FIG. 4.

FIG. 4.

(a) Production of IL-4, IL-5, and IL-10 by thoracic cavity cells. Cells were recovered 28 days p.i., and cytokines were measured after stimulation with L. sigmodontis (LS) adult worm antigen, concanavalin A, or medium only as control. The data show that antigen-specific IL-5 and IL-10 production of cells from EPO−/− mice and BALB/c wild-type mice was significantly higher than that in the other mouse strains. Furthermore, cells of MBP−/− mice showed a higher capacity of IL-10 production than 129/SvJ controls, while IL-5 production was equivalent to that in 129/SvJ wild-type mice. Thoracic cavity cells from 129/SvJ wild-type mice secreted more IL-4 after antigen-specific stimulation compared to MBP−/− and EPO−/−. Cells incubated with medium or concanavalin A show no significant differences between the groups. Each group contained five animals. The data are from one representative experiment out of two. Bars show the mean ± standard deviation. Asterisks represents a significant difference (P < 0.05). ND, not detected. (b) Cytokine production by adherent macrophages from thoracic cavity cells. Stimulation of macrophages with adult worm antigen shows that MBP−/− and EPO−/− macrophages produce significantly higher IL-10 levels than 129/SvJ wild-type mice. EPO−/− macrophages secrete more IL-10 when stimulated with LPS. Each group contains five animals. The data are from one representative experiment out of two. Bars show the mean ± standard deviation. Asterisks represent significant differences (P < 0.05). ND, not detected. (c) Cytokine production by purified CD4+ T cells separated by FACS and stimulated with adult worm antigen or concanavalin A. CD4+ T cells of 129/SvJ wild-type mice show a significantly higher production of IL-4 than those of both knockout strains. IL-5 levels are significantly higher in EPO−/− CD4 T cells compared to the other mouse strains. Each group contains five animals. The data are from one representative experiment out of two. Bars show the mean ± standard deviation. Asterisks represent significant differences (P < 0.05). ND, not detected.