TABLE 1.
opa gene and Opa protein repertoires of 77 hyperinvasive meningococci
| Group and isolate | Year, country of isolation | Phenotype (serogroup: serotype: serosubtype)a | ST |
opaA locusb
|
opaB locusb
|
opaD locusb
|
opaJ locusb
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Allele | SV | HV1 | HV2 | Allele | SV | HV1 | HV2 | Allele | SV | HV1 | HV2 | Allele | SV | HV1 | HV2 | ||||
| ST-1 complex/ subgroup I/II | |||||||||||||||||||
| 6748 | 1971, Canada | A:4,21:P1.3,6 | 1 | 243 | 2-1 | 1-1 | 9-3 | 326 | 2-2 | 18-7 | 14-1 | 77 | 2-2 | 1B-4 | 6-1 | 325 | 2-1 | 11-1 | 1-4 |
| 20 | 1963, Niger | A:4,21:P1.10 | 1 | 252 | 4-2 | 13-1 | 17-7 | 340 | 2-2 | 1A-1 | 8A-1 | 145 | 4-2 | 12-2 | 21-1 | 345 | 2-1 | 11-1 | 1-10 |
| 254 | 1966, Djibouti | A:4,21:P1.10 | 1 | 252 | 4-2 | 13-1 | 17-7 | 333 | 4-2 | 13-1 | 1-10 | 145 | 4-2 | 12-2 | 21-1 | 349 | 4-2 | 1A-1 | 8A-8 |
| 129 | 1964, West Germany | A:4,21:P1.10 | 1 | 250 | 2-1 | 1-4 | 9A-1 | 304 | 2-1 | 1-4 | 9-7 | 311 | 2-2 | 3-8 | 9-4 | 325 | 2-1 | 11-1 | 1-4 |
| 371 | 1980, India | A:4,21:P1.10 | 1 | 243 | 2-1 | 1-1 | 9-3 | 326 | 2-2 | 18-7 | 14-1 | 344 | 2-2 | 18-7 | 14-1 | 346 | 2-2 | 11-1 | 1-4 |
| 139M | 1968, Philippines | A:— | 1 | 96 | 2-1 | 19-10 | 14-3 | 326 | 2-2 | 18-7 | 14-1 | 77 | 2-2 | 1B-4 | 6-1 | 325 | 2-1 | 11-1 | 1-4 |
| 120M | 1967, Pakistan | A:4,21:P1.10 | 1 | 249 | 4-2 | 1-1 | 9-3 | 326 | 2-2 | 18-7 | 14-1 | 77 | 2-2 | 1B-4 | 6-1 | 325 | 2-1 | 11-1 | 1-4 |
| S5611 | 1977, Australia | A:— | 1 | 34 | 4-3 | 18-3 | 14-1 | 298 | 4-2 | 3-2 | 19-2 | 77 | 2-2 | 1B-4 | 6-1 | 325 | 2-1 | 11-1 | 1-4 |
| 106 | 1967, Morocco | A:4,21:P1.10 | 1 | 243 | 2-1 | 1-1 | 9-3 | 326 | 2-2 | 18-7 | 14-1 | 77 | 2-2 | 1B-4 | 6-1 | 325 | 2-1 | 11-1 | 1-4 |
| 393 | 1968, Greece | A:— | 1 | 243 | 2-1 | 1-1 | 9-3 | 326 | 2-2 | 18-7 | 14-1 | 77 | 2-2 | 1B-4 | 6-1 | 325 | 2-1 | 11-1 | 1-4 |
| 322/85 | 1985, East Germany | A:4,21:P1.10 | 2 | 255 | 4-1 | 1A-1 | 8A-6 | 14 | 2-2 | 18-1 | 15-2 | 243 | 2-1 | 1-1 | 9-3 | 208 | 2-2 | 3-2 | 8-A3 |
| 79128 | 1979, China | A:— | 3 | 248 | 4-3 | 16-2 | 5-13 | 327 | 2-2 | 5-8 | 9-6 | 248 | 4-3 | 16-2 | 5-13 | 346 | 2-2 | 11-1 | 1-4 |
| BZ133 | 1977, The Netherlands | B:NT:— | 1 | 243 | 2-1 | 1-1 | 9-3 | 326 | 2-2 | 18-7 | 14-1 | 77 | 2-2 | 1B-4 | 6-1 | 325 | 2-1 | 11-1 | 1-4 |
| 79126 | 1979, China | A:4:P1.7 | 3 | 248 | 4-3 | 16-2 | 5-13 | 341 | 2-1 | 1B-4 | 6-3 | 248 | 4-3 | 16-2 | 5-13 | 346 | 2-2 | 11-1 | 1-4 |
| ST-4 complex/ subgroup IV | |||||||||||||||||||
| A4/M1027 | 1937, United States | A:4,21:— | 4 | 242 | 2-1 | 2-3 | 1-8 | 95 | 2-1 | 1-1 | 9-3 | 310 | 2-1 | 17-3 | 17-8 | 242 | 2-1 | 2-3 | 1-8 |
| 26 | 1963, Niger | A:— | 4 | 242 | 2-1 | 2-3 | 1-8 | 297 | 2-2 | 11-2 | 9B-1 | 296 | 2-1 | 17-3 | 17-8 | NP | NP | NP | NP |
| 243 | 1966, Cameroon | A:— | 4 | 242 | 2-1 | 2-3 | 1-8 | 253 | 2-10 | 1-3 | 8A-5 | 296 | 2-1 | 17-3 | 17-8 | NP | NP | NP | NP |
| 2059001 | 1990, Mali | A:4,21:P1.7 | 4 | 242 | 2-1 | 2-3 | 1-8 | 278 | 2-1 | 2-3 | 9B-1 | 296 | 2-1 | 17-3 | 17-8 | NP | NP | NP | NP |
| 10 | 1963, Burkina Faso | A:— | 4 | 242 | 2-1 | 2-3 | 1-8 | 253 | 2-10 | 1-3 | 8A-5 | 296 | 2-1 | 17-3 | 17-8 | NP | NP | NP | NP |
| 255 | 1966, Burkina Faso | A:4,21:P1.7 | 4 | 242 | 2-1 | 2-3 | 1-8 | 297 | 2-2 | 11-2 | 9B-1 | 296 | 2-1 | 17-3 | 17-8 | NP | NP | NP | NP |
| S3131 | 1973, Ghana | A:— | 4 | 242 | 2-1 | 2-3 | 1-8 | 297 | 2-2 | 11-2 | 9B-1 | 296 | 2-1 | 17-3 | 17-8 | NP | NP | NP | NP |
| 690 | 1980, India | A:4,21:P1.7 | 4 | 254 | 2-1 | 17-3 | 1-8 | 278 | 2-1 | 2-3 | 9B-1 | 315 | 2-1 | 17-3 | 9B-1 | NP | NP | NP | NP |
| C751 | 1983, Gambia | A:— | 4 | 242 | 2-1 | 2-3 | 1-8 | 278 | 2-1 | 2-3 | 9B-1 | 315 | 2-1 | 17-3 | 9B-1 | NP | NP | NP | NP |
| 1014 | 1985, Sudan | A:— | 4 | 242 | 2-1 | 2-3 | 1-8 | 328 | 2-2 | 11-2 | 9B-1 | 296 | 2-1 | 17-3 | 17-8 | NP | NP | NP | NP |
| D8 | 1990, Mali | A:— | 4 | 242 | 2-1 | 2-3 | 1-8 | 278 | 2-1 | 2-3 | 9B-1 | 309 | 2-1 | 17-3 | 9B-1 | NP | NP | NP | NP |
| ST-5 complex/ subgroup III | |||||||||||||||||||
| IAL2229 | 1976, Brazil | A:— | 5 | 242 | 2-1 | 2-3 | 1-8 | 253 | 2-10 | 1-3 | 8A-5 | 296 | 2-1 | 17-3 | 17-8 | 127 | 4-3 | 3-6 | 1-1 |
| 153 | 1966, China | A:4,21:P1.9 | 5 | 242 | 2-1 | 2-3 | 1-8 | 253 | 2-10 | 1-3 | 8A-5 | 301 | 4-3 | 17-3 | 17-8 | 127 | 4-3 | 3-6 | 1-1 |
| 154 | 1966, China | A:4,21:P1.9 | 6 | 242 | 2-1 | 2-3 | 1-8 | 253 | 2-10 | 1-3 | 8A-5 | 296 | 2-1 | 17-3 | 17-8 | 127 | 4-3 | 3-6 | 1-1 |
| 14/1455 | 1970, USSR | A:4,21:P1.20,9 | 5 | 242 | 2-1 | 2-3 | 1-8 | 127 | 4-3 | 3-6 | 1-1 | 296 | 2-1 | 17-3 | 17-8 | 127 | 4-3 | 3-6 | 1-1 |
| S4355 | 1974, Denmark | A:— | 5 | 253 | 2-10 | 1-3 | 8A-5 | 253 | 2-10 | 1-3 | 8A-5 | 296 | 2-1 | 17-3 | 17-8 | 127 | 4-3 | 3-6 | 1-1 |
| 7891 | 1975, Finland | A:4,21:P1.9 | 5 | 242 | 2-1 | 2-3 | 1-8 | 296 | 2-1 | 17-3 | 17-8 | 296 | 2-1 | 17-3 | 17-8 | 127 | 4-3 | 3-6 | 1-1 |
| F4698 | 1987, Saudi Arabia | A:— | 5 | 242 | 2-1 | 2-3 | 1-8 | 253 | 2-10 | 1-3 | 8A-5 | 299 | 2-1 | 17-3 | 17-8 | 127 | 4-3 | 3-6 | 1-1 |
| H1964 | 1987, United Kingdom | A:— | 5 | 242 | 2-1 | 2-3 | 1-8 | 281 | 2-1 | 6-1 | 9-2 | 299 | 2-1 | 17-3 | 17-8 | 127 | 4-3 | 3-6 | 1-1 |
| F6124 | 1988, Chad | A:— | 5 | 242 | 2-1 | 2-3 | 1-8 | 281 | 2-1 | 6-1 | 9-2 | 299 | 2-1 | 17-3 | 17-8 | 127 | 4-3 | 3-6 | 1-1 |
| 92001 | 1992, China | A:— | 7 | 242 | 2-1 | 2-3 | 1-8 | 253 | 2-10 | 1-3 | 8A-5 | 296 | 2-1 | 17-3 | 17-8 | 127 | 4-3 | 3-6 | 1-1 |
| 11-004 | 1984, China | A:— | 5 | 242 | 2-1 | 2-3 | 1-8 | 329 | 2-1 | 2-3 | 8A-5 | 296 | 2-1 | 17-3 | 17-8 | 127 | 4-3 | 3-6 | 1-1 |
| 80049 | 1963, China | A:4:P1.10 | 5 | 242 | 2-1 | 2-3 | 1-8 | 342 | 2-2 | 5-1 | 8C-2 | 296 | 2-1 | 17-3 | 17-8 | 127 | 4-3 | 3-6 | 1-1 |
| ST-8 complex/ cluster A4 | |||||||||||||||||||
| BZ 10 | 1967, The Netherlands | B:2b:P1.2 | 8 | 246 | 2-2 | 15-3 | 12-2 | 277 | 2-2 | 15-3 | 12-2 | 161 | 2-2 | 11-1 | 1-4 | 257 | 4-2 | 15-3 | 12-2 |
| B6116/77 | 1977, Iceland | B:— | 10 | 258 | 4-3 | 19-4 | 14-2 | 277 | 2-2 | 15-3 | 12-2 | 161 | 2-2 | 11-1 | 1-4 | 258 | 4-3 | 19-4 | 14-2 |
| BZ 163 | 1979, The Netherlands | B:2b:P1.16 | 9 | 256 | 2-2 | 15-3 | 12-2 | 343 | 2-2 | 3-2 | 8A-8 | 161 | 2-2 | 11-1 | 1-4 | 256 | 2-2 | 15-3 | 12-2 |
| G2136 | 1986, England | B:— | 8 | 257 | 4-2 | 15-3 | 12-2 | 285 | 2-1 | 19-3 | 2-1 | 161 | 2-2 | 11-1 | 1-4 | 257 | 4-2 | 15-3 | 12-2 |
| SB25 | 1990, South Africa | C:— | 8 | 246 | 2-2 | 15-3 | 12-2 | 246 | 2-2 | 15-3 | 12-2 | 161 | 2-2 | 11-1 | 1-4 | 246 | 2-2 | 15-3 | 12-2 |
| AK22 | 1992, Greece | B:— | 8 | 260 | 2-2 | 3-2 | 6-2 | 294 | 2-2 | 3-2 | 12-2 | 161 | 2-2 | 11-1 | 1-4 | 323 | 4-2 | 3-2 | 12-2 |
| 94/155 | 1994, New Zealand | C:— | 66 | 246 | 2-2 | 15-3 | 12-2 | 292 | 2-1 | 5-2 | 18-1 | 161 | 2-2 | 11-1 | 1-4 | 246 | 2-2 | 15-3 | 12-2 |
| 312 901 | 1996, England | C:— | 8 | 259 | 2-2 | 5-2 | 18-1 | 292 | 2-1 | 5-2 | 18-1 | 161 | 2-2 | 11-1 | 1-4 | 292 | 2-1 | 5-2 | 18-1 |
| ST-11 complex/ ET-37 complex | |||||||||||||||||||
| 38VI | 1964, United States | B:—:P1.5,2 | 11 | 244 | 4-3 | 5-5 | 8C-1 | 34 | 4-3 | 18-3 | 14-1 | 132 | 2-2 | 11-2 | 1-6 | 317 | 2-2 | 19-1 | 14-1 |
| NG P20 | 1969, Norway | B:2a:P1.2 | 11 | 244 | 4-3 | 5-5 | 8C-1 | 279 | 2-2 | 1A-5 | 9B-2 | 132 | 2-2 | 11-2 | 1-6 | 317 | 2-2 | 19-1 | 14-1 |
| F1576 | 1984, Ghana | C:2a:P1.5,2 | 11 | 244 | 4-3 | 5-5 | 8C-1 | 305 | 4-3 | 5-10 | 9B-2 | 132 | 2-2 | 11-2 | 1-6 | 317 | 2-2 | 19-1 | 14-1 |
| 500 | 1984, Italy | C:2a | 11 | 244 | 4-3 | 5-5 | 8C-1 | 300 | 2-2 | 5-9 | 9B-2 | 132 | 2-2 | 11-2 | 1-6 | 317 | 2-2 | 19-1 | 14-1 |
| MA-5756 | 1985, Spain | C:2a:P1.5 | 11 | 244 | 4-3 | 5-5 | 8C-1 | 339 | 4-3 | 5-5 | 8C-1 | 132 | 2-2 | 11-2 | 1-6 | 317 | 2-2 | 19-1 | 14-1 |
| M597 | 1988, Israel | C:2a:P1.5 | 11 | 244 | 4-3 | 5-5 | 8C-1 | 339 | 4-3 | 5-5 | 8C-1 | 132 | 2-2 | 11-2 | 1-6 | 347 | 2-2 | 19-11 | 14-1 |
| D1 | 1989, Mali | C:2a:P1.5 | 11 | 132 | 2-2 | 11-2 | 1-6 | 34 | 4-3 | 18-3 | 14-1 | 132 | 2-2 | 11-2 | 1-6 | 317 | 2-2 | 19-1 | 14-1 |
| 90/18311 | 1990, Scotland | C:NT:P1.5 | 11 | 244 | 4-3 | 5-5 | 8C-1 | 244 | 4-3 | 5-5 | 8C-1 | 132 | 2-2 | 11-2 | 1-6 | 347 | 2-2 | 19-11 | 14-1 |
| L93/4286 | 1993, England | C:— | 11 | 132 | 2-2 | 11-2 | 1-6 | 295 | 4-3 | 10-3 | 10-5 | 132 | 2-2 | 11-2 | 1-6 | 34 | 4-3 | 18-3 | 14-1 |
| BRAZ10 | 1976, Brazil | C:2a:P1.5 | 11 | 244 | 4-3 | 5-5 | 8C-1 | 34 | 4-3 | 18-3 | 14-1 | 316 | 4-2 | 12B-1 | 1-9 | 348 | 2-2 | 3-6 | 1-1 |
| ST-32 complex/ ET-5 complex | |||||||||||||||||||
| 8680 | 1987, Chile | B:15:P1.3 | 32 | 96 | 2-1 | 19-10 | 14-3 | 185 | 5-1 | 10-5 | 3-1 | 147 | 2-2 | 1A-2 | 8-1 | 218 | 2-1 | 19-10 | 14-5 |
| BZ 83 | 1984, The Netherlands | B:NT | 34 | 96 | 2-1 | 19-10 | 14-3 | 335 | 2-1 | 10-5 | 3-1 | 147 | 2-2 | 1A-2 | 8-1 | 218 | 2-1 | 19-10 | 14-5 |
| 204/92 | 1992, Cuba | B:— | 33 | 261 | 2-2 | 19-7 | 14-3 | 185 | 5-1 | 10-5 | 3-1 | 147 | 2-2 | 1A-2 | 8-1 | 322 | 2-2 | 19-7 | 14-5 |
| EG 329 | 1985, East Germany | B:15:P1.16 | 32 | 96 | 2-1 | 19-10 | 14-3 | 185 | 5-1 | 10-5 | 3-1 | 147 | 2-2 | 1A-2 | 8-1 | 218 | 2-1 | 19-10 | 14-5 |
| NG 80 | 1981, Norway | B:15:P1.16 | 32 | 96 | 2-1 | 19-10 | 14-3 | 288 | 5-1 | 1A-2 | 8-1 | 71 | 4-2 | 19-7 | 4-1 | 218 | 2-1 | 19-10 | 14-5 |
| BZ 169 | 1985, The Netherlands | B:NT:P1.16 | 32 | 96 | 2-1 | 19-10 | 14-3 | 185 | 5-1 | 10-5 | 3-1 | 147 | 2-2 | 1A-2 | 8-1 | 218 | 2-1 | 19-10 | 14-5 |
| 44/76 | 1976, Norway | B:— | 32 | 96 | 2-1 | 19-10 | 14-3 | 288 | 5-1 | 1A-2 | 8-1 | 147 | 2-2 | 1A-2 | 8-1 | 218 | 2-1 | 19-10 | 14-5 |
| NG144/82 | 1982, Norway | B:15:P1.16 | 32 | 96 | 2-1 | 19-10 | 14-3 | 313 | 4-2 | 7-1 | 5-11 | 147 | 2-2 | 1A-2 | 8-1 | 218 | 2-1 | 19-10 | 14-5 |
| 196/87 | 1987, Norway | C:15 | 32 | 96 | 2-1 | 19-10 | 14-3 | 185 | 5-1 | 10-5 | 3-1 | 147 | 2-2 | 1A-2 | 8-1 | 218 | 2-1 | 19-10 | 14-5 |
| NG PB24 | 1985, Norway | B:NT:P1.16 | 32 | 96 | 2-1 | 19-10 | 14-3 | 185 | 5-1 | 10-5 | 3-1 | 185 | 5-1 | 10-5 | 3-1 | 218 | 2-1 | 19-10 | 14-5 |
| ST-41/44 complex/ lineage 3 | |||||||||||||||||||
| 931905 | 1993, The Netherlands | B:— | 41 | 265 | 3-2 | 7-1 | 5-9 | 265 | 3-2 | 7-1 | 5-9 | 265 | 3-2 | 7-1 | 5-9 | 213 | 2-1 | 3-6 | 1-1 |
| 50/94 | 1994, Norway | B:— | 45 | 245 | 2-1 | 1-5 | 9A-2 | 293 | 2-1 | 4-2 | 7-3 | 245 | 2-1 | 1-5 | 9A-2 | 213 | 2-1 | 3-6 | 1-1 |
| 88/03415 | 1988, Scotland | B:— | 46 | 245 | 2-1 | 1-5 | 9A-2 | 201 | 2-2 | 4-1 | 7-3 | 265 | 3-2 | 7-1 | 5-9 | 320 | 4-2 | 11-1 | 1-4 |
| 91/40 | 1991, New Zealand | B:4:P1.4 | 42 | 201 | 2-2 | 4-1 | 7-3 | 201 | 2-2 | 4-1 | 7-3 | 308 | 3-2 | 7-1 | 5-9 | 213 | 2-1 | 3-6 | 1-1 |
| AK50 | 1992, Greece | B:— | 41 | 262 | 2-1 | 7-1 | 5-9 | 201 | 2-2 | 4-1 | 7-3 | 303 | 3-2 | 1B-7 | 5-9 | 213 | 2-1 | 3-6 | 1-1 |
| BZ198 | 1986, The Netherlands | B:NT | 41 | 245 | 2-1 | 1-5 | 9A-2 | 264 | 2-1 | 1B-4 | 6-1 | 265 | 3-2 | 7-1 | 5-9 | 213 | 2-1 | 3-6 | 1-1 |
| M-101/93 | 1993, Iceland | B:— | 41 | 263 | 2-2 | 12-1 | 4B-5 | 265 | 3-2 | 7-1 | 5-9 | 213 | 2-1 | 3-6 | 1-1 | 213 | 2-1 | 3-6 | 1-1 |
| M40/94 | 1994, Chile | B:— | 41 | 264 | 2-1 | 1B-4 | 6-1 | 265 | 3-2 | 7-1 | 5-9 | 265 | 3-2 | 7-1 | 5-9 | 132 | 2-2 | 11-2 | 1-6 |
| 400 | 1991, Austria | B:— | 40 | 245 | 2-1 | 1-5 | 9A-2 | 201 | 2-2 | 4-1 | 7-3 | 201 | 2-2 | 4-1 | 7-3 | 213 | 2-1 | 3-6 | 1-1 |
| NG E30 | 1988, Norway | B:4:P1.16 | 44 | 24 | 4-3 | 19-10 | 11-2 | 332 | 2-1 | 3-9 | 8-4 | 24 | 4-3 | 19-10 | 11-2 | 213 | 2-1 | 3-6 | 1-1 |
| NG H15 | 1988, Norway | B:8:P1.15 | 43 | 471 | 2-1 | 4-2 | 7-3 | 142 | 4-6 | 12A-1 | 20-1 | 248 | 4-3 | 16-2 | 5-13 | 346 | 2-2 | 11-1 | 1-4 |
| NG H36 | 1988, Norway | B:8:P1.2 | 47 | 24 | 4-3 | 19-10 | 11-2 | 313 | 4-2 | 7-1 | 5-11 | 313 | 4-2 | 7-1 | 5-11 | 213 | 2-1 | 3-6 | 1-1 |
a
—, serotype or serosubtype not available.
b
Numbers in SV, HV1, and HV2 columns are given according to the following convention: amino acid sequence family-amino acid sequence variant (for example, the first entry in the SV column, 2-1, refers to the first variant of the second amino acid sequence homology family). The most predominant allele at each locus in each clonal complex is indicated in bold. Regions SV, HV1, and HV2 were determined by translation of nucleotide sequences followed by database interrogation. Opa variable region sequences are available at http://neisseria.org/nm/typing/opa/. NP, not present.