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Nineteen Western blots of hyphal protein extracts probed with sera from individual mice after immunization either (A) with HS or (B) through nasopulmonary exposure to VC. HRP-conjugated goat anti-mouse IgG was used for chemiluminescent detection. (C) Western blots of prefractionated HS and CF. HS was passed through a 30-kDa cutoff membrane and was then separated into filtrate and retentate by use of a 10-kDa cutoff membrane.
