FIG. 5.

(A) Survival curves for mice vaccinated with truncated versions of Asp f 3 with TM adjuvant (as indicated by each first and last amino acid residue number) and particulate, adjuvant-free full-length rAsp f 3 (partic. 1-168). Eight animals were used in each group, except for Asp f 3(1-142) with 7 mice and the control group with 11 mice. (B) Serological analysis of pooled plasma from these mice, obtained by Western blotting (around 19 kDa). (C) The Coomassie-stained gel indicates comparable loading amounts of the rAsp f 3 versions (250 ng/band).