TABLE 1.
Constitutive ospC expression results in spirochetal clearance by passively transferred OspC MAb in SCID micea
| Transformant | Missing plasmids | Antibody received | No. of positive cultures/total no. of specimens examined
|
|||
|---|---|---|---|---|---|---|
| Heart | Joint | Skin | All sites | |||
| 5A13/pBBE22 | cp9, lp25, lp56, lp21 | IgG2a | 5/5 | 5/5 | 5/5 | 15/15 |
| OspC MAb | 5/5 | 5/5 | 5/5 | 15/15 | ||
| 5A13/pBBE22-ospC′ | cp9, lp25, lp56, lp21, lp5 | IgG2a | 5/5 | 5/5 | 5/5 | 15/15 |
| OspC MAb | 0/5 | 0/5 | 0/5 | 0/15 | ||
a
Groups of 10 BALB/c SCID mice were inoculated with transformant 5A13/pBBE22 or 5A13/pBBE22-ospC′, passively immunized with either six doses of OspC MAb or mouse IgG2a as a control, and sacrificed 3 days after the last passive transfer. Heart, tibiotarsal joint, and skin specimens were harvested and cultured for spirochetes in BSK-H complete medium.