TABLE 3.
Comparison of Southern blot hybridization and PCR data for gene distribution
| Clone name | Data set |
P. aeruginosa strain
|
No. of positive strains | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Pitt A | Pitt C | Pitt D | Pitt E | Pitt F | 27853 | M18851 | M18858 | W27912 | W27931 | W28869 | PAO1 | |||
| L001_PA_0021_F02 | PCR | + | − | + | + | − | + | − | − | + | + | + | − | 7 |
| Southern blotting | + | − | + | + | − | + | − | − | + | + | + | − | 7 | |
| L001_PA_0031_I05 | PCR | − | − | − | + | − | + | − | − | + | + | − | − | 4 |
| Southern blotting | − | + | − | + | − | + | − | + | + | + | − | − | 6 | |
| L001_PA_0033_J17 | PCR | − | + | + | − | − | − | − | + | + | + | + | − | 6 |
| Southern blotting | + | − | + | − | − | − | + | + | + | + | + | − | 7 | |
| L001_PA_0166_M20 | PCR | + | + | + | − | + | + | − | − | + | − | − | − | 6 |
| Southern blotting | + | + | + | − | − | + | + | + | + | − | + | − | 7 | |
| L001_PA_0304_K14 | PCR | + | + | + | + | + | + | + | + | + | + | + | + | 12 |
| Southern blotting | + | + | + | + | + | + | + | + | + | + | + | + | 12 | |
| L001_PA_0393_J21 | PCR | + | − | + | + | − | + | + | + | + | + | + | − | 9 |
| Southern blotting | + | − | + | + | + | + | + | + | + | + | + | − | 10 | |