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. 2006 Sep;74(9):5200–5210. doi: 10.1128/IAI.00534-06

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FIG. 6. — PLC levels in genotype C and selected genotype A vegetative culture supernatants. Late-log-phase vegetative culture supernatants were assessed to determine their PLC activities using an egg yolk hydrolysis assay. A standard curve was generated using semipurified PLC. PLC activity is shown for genotype C isolates (A) and selected type A isolates (B). PLC results for genotype C isolates are shown for the different toxin subgenotypes (all isolates carry the plc gene). The total numbers of isolates in the PLC activity ranges are indicated in parentheses. ND, toxin could not be detected in culture supernatants.