TABLE 1.
Oligonucleotide primers used for ACE2 and SWI5 deletions in S. cerevisiae
| Primer | Sequencea |
|---|---|
| BMDACE2-F | ATGGATAACGTTGTAGATCCGTGGTATATAAATCCCAGCTGAAGCTTCGTACGC |
| BMDACE2-R | TCAGAGAGCATCAGTTTCGTTTGAAAGGGTGCGGTTGCATAGGCCACTAGTGGATCTG |
| ACE2-TEST-F | CTCAAGCAACAGTTAAAGTGC |
| ACE2-TEST-R | CCACCTCTTCTTGCTCTTCTA |
| KAN-2 | TGCGCCTGAGCGAGACGA |
| KOS-1 | ACAATATAGATACCACCATATAACTCGTTCTTGGAGCTAGCAGCTGAAGCTTCGTACGC |
| KOS-2 | AAAGTGTTCATACTATATTATTTACTTAGATTATACATAAGCATAGGCCACTAGTGGATCTG |
| KOS-3 | AGAGCGAATCAAATATCGCAG |
| KOS-5 | CATAATCCCCGTTCCATTCC |
a
Underlined sequences were derived from the pFA6-kanMX2 multiple cloning site or from the C. glabrata HIS3 gene.