TABLE 2.
Effects of chitinase treatment applied to fungal cell suspensions after growth and during growth on the outcome of intravenous challenge experiments with micea
| Fungal strain | Time of chitinase treatment | Challenge dose (CFU/g) | MST (days)b | MKB (log CFU)c |
|---|---|---|---|---|
| C. glabrata HLS121 | After growth | 2.0 × 106 | 28.0 ± 0.0 | 5.4 ± 0.6 |
| During growth | 2.0 × 106 | 24.0 ± 9.8 | 6.4 ± 1.6 | |
| C. glabrata HLS122 | After growth | 1.5 × 106 | 2.0 ± 0.0 | 5.6 ± 0.7 |
| During growth | 1.5 × 106 | 2.0 ± 0.0 | 6.0 ± 0.8 | |
| C. albicans SC5314 | After growth | 1.6 × 104 | 4.0 ± 2.4 | 5.8 ± 0.9 |
| During growth | 1.6 × 104 | 3.0 ± 0.9 | 6.7 ± 0.4 |
a
All experiments were done with groups of six mice. Mice challenged with C. glabrata isolates were pretreated with cyclophosphamide to induce temporary neutropenia.
b
MST, mean survival time.
c
MKB, mean kidney burden of fungi.