TABLE 1.
Sequences of primers used for PCR amplification
| Primer | Sequence (5′-3′)a |
|---|---|
| E1098A-Fwd | GCT ATA ACA ACT TCA TCA CTG CAG TTA ACA ACC TGA ACA AAC GCA TGG |
| E1098A-Rev | CCA TGC GTT TGT TCA GGT TGT TAA CTG CAG TGA TGA AGT TGT TAT AGC |
| V1099R-Fwd | GCT ATA ACA ACT TCA TCA CTG AAC GTA ACA ACC TGA ACA AAC GCA TGG |
| V1099R-Rev | CCA TGC GTT TGT TCA GGT TGT TAC GTT CAG TGA TGA AGT TGT TAT AGC |
| N1100A-Fwd | CTT CAT CAC TGA AGT TGC CAA CCT GAA CAA ACG CAT GGG CG |
| N1100A-Rev | CGC CCA TGC GTT TGT TCA GGT TGG CAA CTT CAG TGA TGA AG |
| N1101A-Fwd | CTT CAT CAC TGA AGT TAA CGC CCT GAA CAA ACG CAT GGG CG |
| N1101A-Rev | CGC CCA TGC GTT TGT TCA GGG CGT TAA CTT CAG TGA TGA AG |
| L1102R-Fwd | CTT CAT CAC TGA AGT TAA CAA CCG GAA CAA ACG CAT GGG CG |
| L1102R-Rev | CGC CCA TGC GTT TGT TCC GGT TGT TAA CTT CAG TGA TGA AG |
| N1103A-Fwd | GAA GTT AAC AAC CTG GCC AAA CGC ATG GGC GAT TTG AGG |
| N1103A-Rev | CCT CAA ATC GCC CAT GCG TTT GGC CAG GTT GTT AAC TTC |
| K1104A-Fwd | CAC TGA AGT TAA CAA CCT GAA CGC ACG CAT GGG CGA TTT GAG GG |
| K1104A-Rev | CCC TCA AAT CGC CCA TGC GTG CGT TCA GGT TGT TAA CTT CAG TG |
| R1105A-Fwd | GTT AAC AAC CTG AAC AAA GCC ATG GGC GAT TTG AGG GAT ATT AAT GGC G |
| R1105A-Rev | CGC CAT TAA TAT CCC TCA AAT CGC CCA TGG CTT TGT TCA GGT TGT TAA C |
| R1105K-Fwd | GTT AAC AAC CTG AAC AAA AAG ATG GGC GAT TTG AGG GAT ATT AAT GGC |
| R1105K-Rev | GCC ATT AAT ATC CCT CAA ATC GCC CAT CTT TTT GTT CAG GTT GTT AAC |
| M1106A-Fwd | CAA CCT GAA CAA ACG CGC GGG CGA TTT GAG GGA TAT TAA TGG CG |
| M1106A-Rev | CGC CAT TAA TAT CCC TCA AAT CGC CCG CGC GTT TGT TCA GGT TG |
| G1107R-Fwd | CCT GAA CAA ACG CAT GCG CGA TTT GAG GGA TAT TAA TGG CGA AGC C |
| G1107R-Rev | GGC TTC GCC ATT AAT ATC CCT CAA ATC GCG CAT GCG TTT GTT CAG G |
| D1108A-Fwd | CCT GAA CAA ACG CAT GGG CGC TTT GAG GGA TAT TAA TGG CG |
| D1108A-Rev | CGC CAT TAA TAT CCC TCA AAG CGC CCA TGC GTT TGT TCA GG |
| L1109R-Fwd | CGC ATG GGC GAT CGG AGG GAT ATT AAT GGC GAA GCC GG |
| L1109R-Rev | CCG GCT TCG CCA TTA ATA TCC CTC CGA TCG CCC ATG CG |
| R1110A-Fwd | CGC ATG GGC GAT TTG GCG GAT ATT AAT GGC GAA GCC GG |
| R1110A-Rev | CCG GCT TCG CCA TTA ATA TCC GCC AAA TCG CCC ATG CG |
| D1111A-Fwd | GC ATG GGC GAT TTG AGG GCT ATT AAT GGC GAA GCC GGT ACG |
| D1111A-Rev | CGT ACC GGC TTC GCC ATT AAT AGC CCT CAA ATC GCC CAT GC |
a
The mutated nucleotides are in boldface.