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Effect of purified A. actinomycetemcomitans Y4 Omp100 on the expression of hDB2 in HGEC. Purified Omp100 was incubated with HGEC at various concentrations for 12 h. After the total RNA extraction, real-time PCR was performed. The results of the real-time PCR are expressed as a ratio comparing the value at time zero to the value at the data time point. The values represent the means ± standard deviations of triplicate experiments.
