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. 2006 Sep;74(9):5211–5220. doi: 10.1128/IAI.00056-06

FIG. 6.

FIG. 6.

Effect of inhibitors on hBD2 mRNA induction by A. actinomycetemcomitans cells. HGEC were pretreated with PDTC (NF-κB inhibitor; 2 to 50 μM), SB203580 (p38 inhibitor; 0.2 to 5 μM), AG126 (JNK inhibitor, 2 to 50 μM), or PD98059 (ERK inhibitor; 0.4 to 10 μM) for 1 h and then stimulated with heat-inactivated A. actinomycetemcomitans Y4 for 12 h. The total RNA was extracted and analyzed using real-time PCR. Gray bar, without bacterial contact; black bar, with bacterial contact. The results of the real-time PCR are expressed as a ratio in comparison to the value at time zero, and the values represent the means ± standard deviations of triplicate cultures. *, differs significantly (P < 0.01) from cells stimulated with A. actinomycetemcomitans Y4 alone.