FIG. 8.

Effect of inhibitors on hBD2 mRNA expression by Omp100. HGEC were pretreated with PDTC (NF-κB inhibitor; 2 μM), SB203580 (p38 inhibitor; 0.2 μM), AG126 (JNK inhibitor; 2 μM), PD98059 (ERK inhibitor; 0.4 μM), anti-human fibronectin affinity-isolated antibody, anti-integrin affinity-isolated antibody, PP2 (selective Src tyrosine kinase inhibitor), PP3 (negative control for the Src tyrosine kinase inhibitor), and genistein (protein tyrosine kinases inhibitor) with each concentration for 1 h and then stimulated with purified Omp100 (100 ng/ml) for 12 h. The total RNA was extracted and analyzed using real-time PCR. The results of the real-time PCR are expressed as a ratio comparing the value at time zero to the value at the data time point. The values represent the means and ± standard deviations of triplicate experiments. *, differs significantly (P < 0.01) from cells stimulated with Omp100 alone.