FIG. 5.

Cytokine profiles of supernatants from PBMC prestimulated with either heat-killed encapsulated B3501 or heat-killed acapsular CAP67 mutant organisms. PBMC were prestimulated with either heat-killed encapsulated B3501 or heat-killed acapsular CAP67 mutant yeasts for 12 days before addition of live encapsulated organisms. Supernatant samples were taken on days 1, 2, 3, 6, 9, and 12 for cytokine analysis. Results are means ± SE from five independent experiments. Supernatants from PBMC prestimulated with the CAP67 mutant had 6- to 10-fold-higher mean IL-6 levels than those from cells prestimulated with B3501 (P ≤ 0.01 at days 1, 2, 3, and 6).