TABLE 1.
Scores related to histological and immunohistochemical examination of endothelia infected with R. rickettsii and corresponding control tissues
| Treatmenta | Time (h) | Scoreb
|
||||
|---|---|---|---|---|---|---|
| COX-2 | HO-1 | vWF | Rickettsia stain | Hematoxylin and eosin stain | ||
| Control | 4 | + | − | +++ | − | − |
| 1× | 4 | + | − | ++ | + | − |
| 5× | 4 | ++++ | − | ++ | +++ | − |
| Control | 8 | − | − | +++ | − | − |
| 1× | 8 | ++ | + | ++ | + | − |
| 5× | 8 | ++++ | − | + | +++ | − |
| Control | 12 | + | − | +++ | − | − |
| 1× | 12 | ++++ | + | + | + | − |
| 5× | 12 | ++++ | ++ | ++ | +++ | + |
| Control | 24 | + | + | +++ | − | − |
| 1× | 24 | ++ | ++ | ++ | ++ | − |
| 5× | 24 | ++ | ++ | +++ | ++++ | ++ |
a
Cords were perfused with medium alone (control) or medium inoculated with either 5 × 105 PFU/ml (1×) or 2.5 × 106 PFU/ml (5×) of R. rickettsii for the indicated times.
b
−, negative staining; positive staining was rated on a scale of 1 (+ [weakly positive]) to 4 (++++ [strongly positive]).