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Southern blot assay. DNAs from the parent strain (lanes a), the P6 mutant (lanes b), and the complemented P6 mutant (lanes c) were digested with EcoRI. Panels were probed with ∼200-bp biotinylated PCR fragments of the chloramphenicol resistance cassette (left) and the P6 gene (right). Molecular size markers are noted in kilobases on the left.
