FIG. 3.

Results of RT-PCR with the P6 mutant and its parent strain, 49P5H1, as noted at the bottom of the gel. Primers used in reactions corresponding to the P6 gene, the upstream tolB gene, and the downstream hypothetical tRNA/rRNA methyltransferase gene are noted at the top of the gel. Lanes a, purified RNA amplified with reverse transcriptase; lanes b, purified RNA amplified with TaqI polymerase to exclude DNA contamination; lanes c, purified DNA amplified with TaqI polymerase; lanes −, distilled water with reverse transcriptase as a negative control. Molecular size markers are noted in kilobases on the left.