TABLE 1.

Oligonucleotide primers used for this study

Primer	Sequencea	Purpose
CATacc5′	CTCTTCTCTCTCTCTGTCGACGACAGCTGATAGAAACAGAAGCCAC	Amplify and clone chloramphenicol resistance cassette
CATacc3′	ATATATATATATATGTCGACAGGCGTAGCACCAGGCGTTTAAGGGCAC	Amplify and clone chloramphenicol resistance cassette
CAT5′probe	AGTAAGTTGGCAGCATCACCC	Amplify chloramphenicol resistance cassette probe for Southern blotting
CAT3′probe	CATTGAGCAACTGACTGAAATGCC	Amplify chloramphenicol resistance cassette probe for Southern blotting
P65′probe	GATGCTGCAGGAAATGGTGCTG	Amplify P6 gene probe for Southern blotting
P63′probe	ACGTTCATCAGTGTTACCTTC	Amplify P6 gene probe for Southern blotting
pP6spec25′	TCTCTCAAGCTTCGTTTAGCTTTTGCTTCTTCTCG	Amplify P6 gene and flanking region for pP6Spec2 for complementation
pP6spec23′	TGTGTGTGGAATTCTAAGAATGGTAGGTTGTGAGAG	Amplify P6 gene and flanking region for pP6Spec2 for complementation
P6n5′	ATATATGGTACCGATATTTGTTCAGCTGC	Amplify region upstream of P6 gene for construction of mutant
P6n3′acc	ATATATATAGATATGTCGACGATTTCTCCTAAATGAG	Amplify region upstream of P6 gene for construction of mutant
P6c5′acc	ATATATATAGATATGTCGACGTATTTCTAATACTTG	Amplify region downstream of P6 gene for construction of mutant
P6c3′	AGTACAAAGCTTGCTGTAACTGCATAACG	Amplify region downstream of P6 gene for construction of mutant
P65′	TGTAGTTCCTCTAACAACGAT	Amplify P6 gene and RT-PCR of P6 transcript
P63′	GTACGCTAACACTGCACGACG	Amplify P6 gene and RT-PCR of P6 transcript
tolB5′	TCGCTGGCGATGAAGGTACGCATTG	RT-PCR of transcript of tolB upstream of P6 gene
tolB3′	CATCATAATCTGCTACACGAA	RT-PCR of transcript of tolB upstream of P6 gene
RNAmtrans5′	TGTTGGAAAATATTCGTATTG	RT-PCR of transcript of HI0380 downstream of P6 gene
RNAmtrans3′	CGTTTTTCAACCGCACTTAGC	RT-PCR of transcript of HI0380 downstream of P6 gene

^aRestriction enzyme sites are underlined.