TABLE 1.
Densitometric analysis of the immunoreactive bands of a Western blot of hCAP-18/LL-37 of exocytosed material of PMNs isolated from PLS patients and their parents of family Aa
| Secretagogue | Mean amt (%) ± SDb
|
|||||
|---|---|---|---|---|---|---|
| hCAP-18
|
Intermediate-size fragments
|
LL-37
|
||||
| Parents | Patients | Parents | Patients | Parents | Patients | |
| fMLP | 46.0 ± 0.1 | 65.9 ± 5.4 | 15.4 ± 3.7 | 27.2 ± 9.6 | 38.6 ± 6.4 | 6.9 ± 4.2 |
| PMA | 48.1 ± 4.7 | 61.0 ± 0.3 | 13.6 ± 0.1 | 28.6 ± 3.4 | 38.3 ± 4.7 | 10.4 ± 3.8 |
| Ionomycin | 40.5 ± 0.1 | 46.7 ± 1.1 | 10.3 ± 0.7 | 20.6 ± 6.11 | 49.2 ± 0.8 | 32.7 ± 5.1 |
a
See Fig. 2A.
b
The total amounts of immune reactive protein were determined by densitometry. Individual bands for hCAP-18, intermediate fragments, and LL-37 are expressed as percent values relative to the total immunoreactive protein (100%). The total immunoreactive protein was expressed per secretagogue (fMLP, PMA, and ionomycin). n = 2 for both parents and patients. Due to the small sample numbers in the groups, the differences were not compared statistically.