FIG. 1.

S. aureus-induced TLR activation in IL-8/TLR reporter cells. A human embryonic kidney (H-K) cell line expressing TLR2, TLR3, or TLR4 was incubated overnight with specific ligand Pam3Cys (TLR2), poly(I:C) (TLR3), or LPS (TLR4) or with S. aureus (number of bacteria/well). IL-8 production was measured by ELISA and presented as a ratio of stimulated to unstimulated cells. Data represent the means of three wells per experimental group. The experiment was repeated three times with similar results.