TABLE 2.

Oligonucleotide primers^a

Primer set	5′ Primer sequenceb	3′ Primer sequence	Purpose of primers
igaBscreen	TGCCCGTTACCGGATAAATG	AACCACGGGCACAAACAGCC	Amplify 1,650-bp region of igaB in screen of 298 strains
igaB2screen	TTCTTCGCCAAAGAAACCGC	ATCTATAAAAAAGAATTTGC	Amplify 1,011-bp region of igaB in screen of 298 strains
igaup	AAAGAATTCAAGTGCGGTAATTTTATTGCACTTACTGTC	AAAGGTACCGCCCAGTAATCATAAGAACC	Amplify and clone 5′ 1 kb of iga gene
igadown	AAAGGTACCAATAACTGATAATTCAGAAAG	AAATCTAGAAAGTGCGGTCTGCAGTTTTTTGTTTTTCCG	Amplify and clone 3′ 1 kb of iga gene
igaBup	AAAGAATTCAAGTGCGGTGCGGGCTTTTATCTTAATGC	AAAGGATCCTTATACCATTATTATCTTAAC	Amplify and clone 1 kb upstream of igaB gene
igaBdown	AAAGGATCCATGATTACAATTAAGAAAGG	AAACTGCAGAAGTGCGGTCTTTTTCATTACCTTCAGCG	Amplify and clone 1 kb downstream of igaB gene
igaprobe	AACGCCACCAATGTATCTGG	TATTTAAATGAATATGCCCC	Amplify iga gene probe for Southern blots
igaBprobe	AACACGATAACGCCGGCACC	AAAAACAGACCGCCCGCACC	Amplify igaB gene probe for Southern blots
specprobe	AAAAAATAGGAGATAAAAGC	TTTTCGTTTTGCTTGGTAAAGC	Amplify spectinomycin cassette probe for Southern blots
catprobe	AGTAAGTTGGCAGCATCACCC	CATTGAGCAACTGACTGAAATGCC	Amplify chloramphenicol cassette probe for Southern blots

^aThe sequence of other oligonucleotide primers referred to in the text are available upon request.

^bRestriction enzyme sites are underlined; H. influenzae uptake sequences are italicized.